Journal: Infection and Drug Resistance

Article Title: Synergistic Effect and Mechanism of Plumbagin with Gentamicin Against Carbapenem-Resistant Klebsiella pneumoniae

doi: 10.2147/IDR.S265753

Figure Lengend Snippet: Plumbagin potentiates antibacterial activity of gentamicin against CRKp. ( A ) Susceptibility test to imipenem of antibiotics-susceptible strain of Klebsiella pneumoniae CCTCC AB 2010163 (left) and CRKp strain ATCC BAA-1705 (right) on LB agar plates. ( B ) Susceptibility test to gentamicin of ATCC BAA-1705 (left) and its induced gentamicin-resistant strain (right) on LB agar plates. ( C ) Heatmap profile of synergistic effect of herbal compounds with gentamicin. Red, no synergistic effect; green, low synergistic effect; and purplish red, high synergistic effect. ( D ) The inhibition of combination treatments against the CRKp. ( E ) The inhibition of combination treatments against antibiotics-susceptible strain of Klebsiella pneumoniae . ( F ) Growth curves of CRKp with different treatments. * P <0.05, ** P <0.01, *** P <0.001, and **** P <0.0001 compared with gentamicin monotherapy.

Article Snippet: The antibiotics-susceptible strain of Klebsiella pneumoniae CCTCC AB 2010163 was obtained from China Center for Type Culture Collection (CCTCC), Wuhan, China.

Techniques: Activity Assay, Inhibition

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Uncovering the link between the SpnIII restriction modification system and LuxS in Streptococcus pneumoniae meningitis isolates

doi: 10.3389/fcimb.2023.1177857

Figure Lengend Snippet: S. pneumoniae strains and oligonucleotide primers used in this study.

Article Snippet: The wildtype S. pneumoniae strains used in this study ( ) were provided to us by the German National Reference Center for Streptococci (Aachen) ( ).

Techniques: Sequencing, Mutagenesis

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Uncovering the link between the SpnIII restriction modification system and LuxS in Streptococcus pneumoniae meningitis isolates

doi: 10.3389/fcimb.2023.1177857

Figure Lengend Snippet: spnIII allele frequencies of 60B and 60CSF recovered post intranasal murine infection. Mice were intranasally challenged with 10 8 CFU S. pneumoniae serotype 15C ST8711 blood isolate (60B) or CSF isolate (60CSF). At 24 h, mice from each group were humanely euthanized and pneumococci in the nasal tissue were harvested. spnIII allele quantification was performed on DNA extracted from the original inoculum and colonies grown from nasopharynx samples. Allele percentages for original inoculum and bacteria recovered from noses are represented. Statistical analysis was performed using two-way analysis of variance and a Tukey post-comparison test; *P < 0.05.

Article Snippet: The wildtype S. pneumoniae strains used in this study ( ) were provided to us by the German National Reference Center for Streptococci (Aachen) ( ).

Techniques: Infection, Bacteria, Comparison

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Synthesis and delivery of Streptococcus pneumoniae capsular polysaccharides by recombinant attenuated Salmonella vaccines

doi: 10.1073/pnas.2013350118

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: STREP14 , Wild-type S. pneumoni ae CPS14 strain , BEI Resources.

Techniques: Recombinant, Plasmid Preparation